|
KMID : 0043320090320091301
|
|
Archives of Pharmacal Research
2009 Volume.32 No. 9 p.1301 ~ p.1308
|
|
|
Timosaponin B-II Inhibits Pro-inflammatory Cytokine Induction by Lipopolysaccharide in BV2 Cells
|
|
Chen Wan-Sheng
Lu Wen-Quan
Tao Xia
Sun Lian-Na
Qiu Yan
Li Tie-Jun
|
|
|
Abstract
|
|
|
|
It was reported that the total polysaccharides extracts from Anemarrhenae asphodeloides Bge(Liliaceae, rhizome) could inhibit inflammatory responses in various models. In the present study, the effects of Timosaponin B-II, a purified extract from A. asphodeloidesb, on the expressions of IL-1β, TNF-α and IL-6, the activity of NF-κB and the activation of signal pathway related to NF-κB were explored in vitro. Timosaponin B-II significantly attenuated increase of these cytokines on both mRNA and protein levels from LPS-stimulated BV2 cells in a dose-dependent manner. The reporter gene assay also showed that the activation of NF-κB induced by LPS was inhibited by pre-treatment with Timosaponin B-II. Moreover, western blot results showed that the activation of p38, JNK and P65 had been decreased. These results suggest that both NF-κB signal pathway and MAPK pathway were involved in the inhibitory effects of Timosaponin B-II on the expression of pro-inflammatory cytokines.
|
|
|
KEYWORD
|
|
|
Timosaponin B-II, Cytokine, NF-κB, MAPK
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|